Beilstein J. Org. Chem.2013,9, 2168–2179, doi:10.3762/bjoc.9.254
was achieved at 20 minute residence time. Finally, the productivity of the reactor was calculated, extrapolated to parallel run units, and compared with data collected previously.
Keywords: Eupergit; flow chemistry; immobilized enzyme; threoninealdolase; Introduction
Enzymes are bio-based catalysts
which is useful to reduce the cost of the process by reusing the catalyst.
Conclusion
To the best of our knowledge, we report the first use of immobilized threoninealdolase in a microreactor for the flow synthesis of phenylserine. So far, we achieved a maximum of about 30% yield under the typical flow
Materials
The enzyme L-allo-threoninealdolase (L-low-TA) (EC 4.1.2.48) having concentration of 3 mg/mL and activity of 0.135 U/mg has a strong preference for L-allo-threonine from Thermotoga maritima and was kindly donated by the Junior Research Group ‘‘Industrial Biotechnology’’ (University of Leipzig